supported lipid membranes

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lipids on pdms

  As another part of my graduate work, I collaborated closely with Peter Lenz, an then-iterant theorist, on his first foray into experimental science. Together we discovered how to tune the surface chemistry of poly(dimethylsiloxane) (PDMS) such that supported bilayer membranes, supported lipid monolayers, intact vesicles, or no lipids would spontaneously adsorb to the surface. The paper describing our observations appeared in Langmuir. Subsequently, Atul Parikh, Andy Shreve, and co-workers made very similar observations in self-assembled monolayers of n-octadecylsiloxanes. 

  In yet another area, I worked with Jennifer Hovis on characterizing interactions between lipid components believed to form lipid rafts. This work indicates that sphingomylein and cholesterol preferentially associate with the ganglioside GM1 in a mainly egg PC lipid bilayer. This preferential association is observed as correlated motion of the sphingomylein and cholesterol with GM1, not as formation of distinct lipid phases. It is also notable that in these experiments none of the components of interest (i.e., GM1, sphingomylein, or cholesterol) were fluorescently labeled - thus avoiding a significant perturbation to the molecular interactions we were interested in studying.  
  These observations provided the stimulus for moving to another technique, high resolution secondary ion mass spectometry, to more directly bilayer structure with only minimal perturbation to any of the membrane components. This is work that Mary Kraft and Carine Galli-Marxer in Steve's lab in collaboration with Peter Weber and Ian Hutcheon at Lawrence Livermore National Labs have pursued to beautiful results (and you should really check it out.)
 the virtual ajo-franklin
 my professional interests
   synthetic networks in budding yeast
   high resolution fluorescence microscopy
   supported lipid membranes
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