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As another part of my graduate work, I collaborated closely with Peter Lenz, an then-iterant theorist, on his
first foray into experimental science. Together we discovered how to
tune the surface chemistry of poly(dimethylsiloxane) (PDMS) such that
supported bilayer membranes, supported lipid monolayers, intact
vesicles, or no lipids would spontaneously adsorb to the surface. The
paper describing our observations appeared in Langmuir.
Subsequently, Atul Parikh, Andy Shreve, and co-workers made very
similar observations in self-assembled monolayers of
n-octadecylsiloxanes.
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In yet another area, I worked with Jennifer Hovis on characterizing
interactions between lipid components believed to form lipid rafts.
This work indicates that sphingomylein and cholesterol preferentially
associate with the ganglioside GM1 in a mainly
egg PC lipid bilayer. This preferential association is observed as
correlated motion of the sphingomylein and cholesterol with GM1,
not as formation of distinct lipid phases. It is also notable that in
these experiments none of the components of interest (i.e., GM1,
sphingomylein, or cholesterol) were fluorescently labeled - thus
avoiding a significant perturbation to the molecular interactions we
were interested in studying.
These observations provided the
stimulus for moving to another technique, high resolution secondary ion
mass spectometry, to more directly bilayer structure with only
minimal perturbation to any of the membrane components. This is
work that Mary Kraft and Carine Galli-Marxer in Steve's lab in
collaboration with Peter Weber and Ian Hutcheon at Lawrence Livermore
National Labs have pursued to beautiful results (and you
should really check it out.) |